TrpRS Monoclonal antibody, PBS Only

TrpRS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C3

Cat no : 67952-1-PBS

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

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Validation Data Gallery

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  WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67952-1-Ig (same clone as 67952-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67952-1-Ig (same clone as 67952-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67952-1-Ig (same clone as 67952-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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Product Information

67952-1-PBS targets TrpRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TrpRS fusion protein Ag31430
• Full Name: tryptophanyl-tRNA synthetase
• Calculated Molecular Weight: 471 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC017489
• Gene Symbol: WARS1
• Gene ID (NCBI): 7453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).