CoraLite® Plus 647-conjugated Vimentin Recombinant antibody

• Featured Product
• KD/KO Validated

Vimentin Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

6K21

Cat no : CL647-80232

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

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Validation Data Gallery

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  IF Staining of HeLa using CL647-80232

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 Vimentin antibody (CL647-80232, Clone: 6K21 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL647-80232

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 Vimentin antibody (CL647-80232, Clone: 6K21 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80232 targets Vimentin in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Vimentin fusion protein Ag0489
• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).