|Positive IF detected in
|Positive FC detected in
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
CL594-60330 targets Vimentin in IF, FC(Intra) applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Mouse / IgG1
|Vimentin fusion protein Ag0489
|Calculated molecular weight
|466 aa, 54 kDa
|GenBank accession number
|Gene ID (NCBI)
|CoraLite®594 Fluorescent Dye
|Excitation/Emission maxima wavelengths
|588 nm / 604 nm
|Protein G purification
|PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
|Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
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