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Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3H9D1

Cat no : 60330-1-Ig

Synonyms

VIM, Vimentin



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, ROS1728 cells, Jurkat cells, A549 cells, U2OS cells, U-251 cells
Positive IHC detected inhuman appendicitis tissue, human renal cell carcinoma tissue, human endometrial cancer tissue, human ovary tumor tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue, human tonsillitis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, Retinal organoids, HepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60330-1-Ig targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
RRIDAB_2881439
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Protocols

Product Specific Protocols
WB protocol for Vimentin antibody 60330-1-IgDownload protocol
IHC protocol for Vimentin antibody 60330-1-IgDownload protocol
IF protocol for Vimentin antibody 60330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
humanWB,IP, IF

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan
mouseWB

Acta Pharm Sin B

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.

Authors - Piao Luo
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
zebrafishIF

Aging Cell

Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.

Authors - Raquel R Martins
mouseWB

Aging Cell

Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.

Authors - Yongxin Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

  • Applications: Immunofluorescence
  • Cell Tissue Type: Retinal organoids
FH

Aarushi (Verified Customer) (04-15-2023)

Primary antibody worked well after methanol treatment in a dilution of 1:200.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Tanycyte monolayer cell cultures (mouse)
Vimentin Antibody Immunofluorescence validation (1:200 dilution) in Tanycyte monolayer cell cultures (mouse) (Cat no:60330-1-Ig)
FH

Saba (Verified Customer) (03-03-2022)

The antibody worked perfectly!! The band was sharp and no unspecefic bands were seen.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF10A cells