Vimentin Monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP50158-1

  Sandwich ELISA standard curve of MP50158-1, Human Vimentin Monoclonal Matched Antibody Pair - PBS only. 60330-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0489. 60330-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP50158-1

  The mean Vimentin concentration was determined to be 3,886.65 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load.

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  Cytometric bead array standard curve of MP50158-1

  Cytometric bead array standard curve of MP50158-1, Vimentin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60330-2-PBS. Detection antibody: 60330-1-PBS. Standard:Ag0489. Range: 3.125-100 ng/mL.

Vimentin Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 60330-2-PBS

Clone No.1F9D8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

VIM, 1F9D8

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Product Information

60330-2-PBS targets Vimentin as part of a matched antibody pair:

MP50158-1: 60330-2-PBS capture and 60330-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vimentin fusion protein Ag0489
• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• ENSEMBL Gene ID: ENSG00000026025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P08670
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.