Vegfa Recombinant antibody

Vegfa Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2I18

Cat no : 82864-7-RR

Synonyms

Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

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Validation Data Gallery

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  IF Staining of NIH/3T3 using 82864-7-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Vegfa antibody (82864-7-RR, Clone: 2I18 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82864-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82864-7-RR against Mouse Vegfa were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:220-1:880

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82864-7-RR targets Vegfa in IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: vascular endothelial growth factor A
• GenBank Accession Number: NM_001287056
• Gene Symbol: Vegfa
• Gene ID (NCBI): 22339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.