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WB analysis of human plasma using 66398-1-Ig (same clone as 66398-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
WB analysis of pig plasma using 66398-1-Ig (same clone as 66398-1-PBS)
pig plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
WB analysis of rabbit serum using 66398-1-Ig (same clone as 66398-1-PBS)
rabbit serum were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66398-1-Ig (same clone as 66398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:350 (under 10x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66398-1-Ig (same clone as 66398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66398-1-Ig (same clone as 66398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66398-1-Ig (same clone as 66398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 66398-1-Ig (same clone as 66398-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VTN antibody (66398-1-Ig, Clone: 1D2C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

VTN Monoclonal antibody, PBS Only

VTN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66398-1-PBS

Clone No.1D2C8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66398-1-PBS targets VTN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rabbit, mouse samples.

Tested Reactivity	human, pig, rabbit, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	VTN fusion protein Ag8443 Predict reactive species
Full Name	vitronectin
Calculated Molecular Weight	478 aa, 54 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC005046
Gene Symbol	VTN
Gene ID (NCBI)	7448
RRID	AB_2881772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P04004
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).