VTN Monoclonal antibody

VTN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D2C8

Cat no : 66398-1-Ig

Synonyms

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN

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Validation Data Gallery

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  WB analysis of human plasma using 66398-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig plasma using 66398-1-Ig

  pig plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit serum using 66398-1-Ig

  rabbit serum were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66398-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:350 (under 10x lens).

• 

  IHC staining of human testis using 66398-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66398-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66398-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 66398-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VTN antibody (66398-1-Ig, Clone: 1D2C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: human plasma tissue, pig plasma tissue, rabbit serum tissue
• Positive IHC detected in: human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66398-1-Ig targets VTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit, mouse samples.

• Tested Reactivity: human, pig, rabbit, mouse
• Cited Reactivity: pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VTN fusion protein Ag8443
• Full Name: vitronectin
• Calculated Molecular Weight: 478 aa, 54 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC005046
• Gene Symbol: VTN
• Gene ID (NCBI): 7448
• RRID: AB_2881772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Publications

Species	Application	Title
pig	IHC	Int J Mol Sci; Large-Scale Protein Analysis of Experimental Retinal Artery Occlusion; Authors - Nanna Vestergaard