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VPS28 Recombinant antibody, PBS Only

VPS28 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241886D3

Cat no : 84472-4-PBS

Synonyms

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, 241886D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS28 Recombinant antibody (84472-4-RR)

    WB analysis using 84472-4-RR (same clone as 84472-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84472-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS28 Recombinant antibody (84472-4-RR)

    IF Staining of HepG2 using 84472-4-RR (same clone as 84472-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84472-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84472-4-RR

    Affinity and Kinetic Characterization of 84472-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84472-4-RR against Human VPS28 were performed. The affinity constant is 1.72 nM.

Product Information

84472-4-PBS targets VPS28 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VPS28 fusion protein Ag7784
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006485
Gene Symbol VPS28
Gene ID (NCBI) 51160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.