CoraLite®594-conjugated VPRBP Monoclonal antibody

VPRBP Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A7A8

Cat no : CL594-66392

Synonyms

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66392

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 VPRBP antibody (CL594-66392, Clone: 1A7A8 ) at dilution of 1:200.

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  FC experiment of K-562 using CL594-66392

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human VPRBP (CL594-66392, Clone:1A7A8) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66392 targets VPRBP in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: VPRBP fusion protein Ag2184
• Full Name: Vpr (HIV-1) binding protein
• Calculated Molecular Weight: 1506 aa, 169 kDa
• Observed Molecular Weight: 169 kDa
• GenBank Accession Number: BC022792
• Gene Symbol: VPRBP
• Gene ID (NCBI): 9730
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).