VLGR1 Polyclonal antibody

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  WB analysis using 31628-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 31628-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 31628-1-AP

  IP result of anti-VLGR1 (IP:31628-1-AP, 4ug; Detection:31628-1-AP 1:500) with SH-SY5Y cells lysate 1920 ug.

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  IF Staining of K-562 using 31628-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using VLGR1 antibody (31628-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

VLGR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 31628-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

GPR98, USH2B, MASS1, KIAA0686, G protein coupled receptor 98

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Tested Applications

• Positive WB detected in: HEK-293T cells, SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31628-1-AP targets VLGR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VLGR1 fusion protein Ag34635
• Full Name: G protein-coupled receptor 98
• Calculated Molecular Weight: 693 kDa
• Observed Molecular Weight: 217 kDa
• GenBank Accession Number: NM_032119
• Gene Symbol: GPR98
• Gene ID (NCBI): 84059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8WXG9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VLGR1 (very large G protein-coupled receptor-1), also known as ADGRV1, GPR98, and MASS, has a molecular weight of up to 700 kDa, making it the largest member of the 33 adhesive G protein-coupled receptors (ADGRs), a unique subfamily of the GPCR superfamily. The full-length VLGR1 encompasses ∼6300 amino acids and thus is difficult to analyze by electrophoresis. Vlgr1 is a core component of ankle-link complex in inner ear hair cells. Knock-out and mutation mouse models show that loss of Vlgr1 function leads to abnormal stereociliary development and hearing loss, indicating crucial roles of Vlgr1 in hearing transduction or auditory system development (PMID: 23180093). Western blot analysis detected VLGR1a isoform at an apparent molecular mass of 217 kDa .