FC experiment of human peripheral blood leukocytes using 98109-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human peripheral blood leukocytes using 98109-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
Affinity and Kinetic Characterization of 98109-1-RR
Biolayer interferometry (BLl) kinetic assays of 98109-1-RR against Human VISTA were performed. The affinity constant is 0.933 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98109-1-RR targets VISTA in FC applications and shows reactivity with human samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.
FC experiment of human peripheral blood leukocytes using 98109-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human peripheral blood leukocytes using 98109-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98109-1-RR
Biolayer interferometry (BLl) kinetic assays of 98109-1-RR against Human VISTA were performed. The affinity constant is 0.933 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
VISTA Recombinant antibody
Catalog Number
98109-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
