VDR Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

VDR Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9C1

Cat no : 67192-1-PBS

Synonyms

NR1I1, VDR, Vitamin D3 receptor

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Validation Data Gallery

• 

  WB analysis using 67192-1-Ig (same clone as 67192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67192-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67192-1-Ig (same clone as 67192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67192-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67192-1-Ig (same clone as 67192-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67192-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67192-1-Ig (same clone as 67192-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67192-1-PBS in a different storage buffer formulation.

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Product Information

67192-1-PBS targets VDR in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: VDR fusion protein Ag28188
• Full Name: vitamin D (1,25- dihydroxyvitamin D3) receptor
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC060832
• Gene Symbol: VDR
• Gene ID (NCBI): 7421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The vitamin D receptor (VDR), also known as NR1I1 (nuclear receptor subfamily 1, group I, member 1), is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or trans-repression of specific gene products.It is an intracellular hormone receptor that specifically binds 1,25(OH)2D3 and mediates its effects. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A). A disorder of vitamin D metabolism results in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. The VDR exists two isoform with the MV 48 kDa and 54 kDa.