VDR Monoclonal antibody

• Featured Product
• KD/KO Validated

VDR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

19

CloneNo.

1A9C1

Cat no : 67192-1-Ig

Synonyms

NR1I1, VDR, Vitamin D3 receptor

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Validation Data Gallery

• 

  WB analysis using 67192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67192-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67192-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67192-1-Ig (VDR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HSC-T6 cells, T-47D cells, NCCIT cells, COLO 320 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Product Information

67192-1-Ig targets VDR in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: VDR fusion protein Ag28188
• Full Name: vitamin D (1,25- dihydroxyvitamin D3) receptor
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC060832
• Gene Symbol: VDR
• Gene ID (NCBI): 7421
• RRID: AB_2882487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vitamin D receptor (VDR), also known as NR1I1 (nuclear receptor subfamily 1, group I, member 1), is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or trans-repression of specific gene products.It is an intracellular hormone receptor that specifically binds 1,25(OH)2D3 and mediates its effects. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A). A disorder of vitamin D metabolism results in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. The VDR exists two isoform with the MV 48 kDa and 54 kDa.

Publications

Species	Application	Title
mouse	WB	Antioxidants (Basel); Fibroblast Upregulation of Vitamin D Receptor Represents a Self-Protective Response to Limit Fibroblast Proliferation and Activation during Pulmonary Fibrosis; Authors - Juan Wei
human	WB	Front Pharmacol; Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway; Authors - Xiaowei Sun
	IF	Front Cell Dev Biol; Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.; Authors - Shanhe Wang
human	WB,IF	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng
mouse	WB	Phytomedicine; Asperuloside inhibited epithelial-mesenchymal transition in colitis associated cancer via activation of vitamin D receptor.; Authors - Yingyu Lu ; KD Validated
human	WB	Front Microbiol; Vitamin D3 Suppresses Human Cytomegalovirus-Induced Vascular Endothelial Apoptosis via Rectification of Paradoxical m6A Modification of Mitochondrial Calcium Uniporter mRNA, Which Is Regulated by METTL3 and YTHDF3.; Authors - Wenbo Zhu ; KD Validated