Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 82463-1-RR
IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug.
IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug.
IHC staining of rat small intestine using 82463-1-RR
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat brain using 82463-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 82463-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Affinity and Kinetic Characterization of 82463-1-RR
Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.
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HeLa cells, A549 cells, MCF-7 cells, RAW 264.7 cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in
rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
rat brain tissue
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82463-1-RR targets VCP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).
Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat small intestine using 82463-1-RR
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 82463-1-RR
IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug.
IF-P Figures
IF Staining of rat brain using 82463-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 82463-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
AFFINITY Figures
Affinity and Kinetic Characterization of 82463-1-RR
Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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