VCP Recombinant antibody

VCP Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G23

Cat no : 82463-1-RR

Synonyms

HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit

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Validation Data Gallery

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  WB analysis using 82463-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82463-1-RR

  IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug.

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  IHC staining of rat small intestine using 82463-1-RR

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82463-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, MCF-7 cells, RAW 264.7 cells, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82463-1-RR targets VCP in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VCP fusion protein Ag1002
• Full Name: valosin-containing protein
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC007562
• Gene Symbol: VCP
• Gene ID (NCBI): 7415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).