VCP Recombinant antibody, PBS Only

VCP Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G23

Cat no : 82463-1-PBS

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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Validation Data Gallery

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  WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

  IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82463-1-PBS targets VCP in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VCP fusion protein Ag1002
• Full Name: valosin-containing protein
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC007562
• Gene Symbol: VCP
• Gene ID (NCBI): 7415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).