VCP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

VCP Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B10

Cat no : 60316-1-PBS

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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Validation Data Gallery

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  WB analysis of HeLa using 60316-1-Ig (same clone as 60316-1-PBS)

  WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 60316-1-Ig (same clone as 60316-1-PBS)

  U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 60316-1-Ig incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60316-1-Ig (same clone as 60316-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 60316-1-Ig (same clone as 60316-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60316-1-Ig (same clone as 60316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60316-1-Ig (same clone as 60316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 60316-1-Ig (same clone as 60316-1-PBS)

  Immunofluorescent analysis of RAW 264.7 cells using 60316-1-Ig (VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60316-1-Ig (same clone as 60316-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (60316-1-Ig, Clone: 2A4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 60316-1-Ig (same clone as 60316-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60316-1-Ig(VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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  FC experiment of HL-60 using 60316-1-Ig (same clone as 60316-1-PBS)

  1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (60316-1-Ig, Clone:2A4B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60316-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60316-1-PBS targets VCP in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VCP fusion protein Ag1002
• Full Name: valosin-containing protein
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC007562
• Gene Symbol: VCP
• Gene ID (NCBI): 7415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).