Filter: AllWBIPIHC

WB analysis using 16364-1-AP (same clone as 16364-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 16364-1-AP (same clone as 16364-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IP experiment of K-562 using 16364-1-AP (same clone as 16364-1-PBS)
IP result of anti-VAV1 (IP:16364-1-AP, 4ug; Detection:16364-1-AP 1:4000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16364-1-AP (same clone as 16364-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16364-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

VAV1 Polyclonal antibody, PBS Only

VAV1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16364-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

VAV, Proto-oncogene vav, Proto oncogene vav

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16364-1-PBS targets VAV1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VAV1 fusion protein Ag9049 Predict reactive species
Full Name	vav 1 guanine nucleotide exchange factor
Calculated Molecular Weight	845 aa, 98 kDa
Observed Molecular Weight	98 kDa
GenBank Accession Number	BC013361
Gene Symbol	VAV1
Gene ID (NCBI)	7409
RRID	AB_2213571
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15498
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).