VARS2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15776-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 15776-1-AP (same clone as 15776-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 15776-1-AP (same clone as 15776-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15776-1-AP (same clone as 15776-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15776-1-AP (same clone as 15776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

  IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 15776-1-AP (same clone as 15776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15776-1-AP (VARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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Product Information

15776-1-PBS targets VARS2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VARS2 fusion protein Ag8460
• Full Name: valyl-tRNA synthetase 2, mitochondrial (putative)
• Calculated Molecular Weight: 119 kDa, 57 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC008844
• Gene Symbol: VARS2
• Gene ID (NCBI): 57176
• RRID: AB_2272699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.