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  • Featured Product
  • KD/KO Validated

VARS2 Polyclonal antibody

VARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15776-1-AP

Synonyms

KIAA1885, Valine tRNA ligase, ValRS, Valyl tRNA synthetase like, VARS2, VARS2L, VARSL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VARS2 Polyclonal antibody (15776-1-AP)

    WB analysis using 15776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VARS2 Polyclonal antibody (15776-1-AP)

    WB analysis using 15776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using VARS2 Polyclonal antibody (15776-1-AP)

    WB analysis of human brain using 15776-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VARS2 Polyclonal antibody (15776-1-AP)

    WB analysis of HeLa using 15776-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using VARS2 Polyclonal antibody (15776-1-AP)

    WB analysis of human liver using 15776-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using VARS2 Polyclonal antibody (15776-1-AP)

    WB analysis of Jurkat using 15776-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using VARS2 Polyclonal antibody (15776-1-AP)

    IP experiment of HeLa using 15776-1-AP

    IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VARS2 Polyclonal antibody (15776-1-AP)

    IHC staining of human liver cancer using 15776-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VARS2 Polyclonal antibody (15776-1-AP)

    IHC staining of human liver cancer using 15776-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VARS2 Polyclonal antibody (15776-1-AP)

    IHC staining of human ovary tumor using 15776-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using VARS2 Polyclonal antibody (15776-1-AP)

    IHC staining of human ovary tumor using 15776-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VARS2 Polyclonal antibody (15776-1-AP)

    IF Staining of HeLa using 15776-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15776-1-AP (VARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human liver tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15776-1-AP targets VARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VARS2 fusion protein Ag8460
Full Name valyl-tRNA synthetase 2, mitochondrial (putative)
Calculated Molecular Weight 119 kDa, 57 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC008844
Gene Symbol VARS2
Gene ID (NCBI) 57176
RRIDAB_2272699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.

Protocols

Product Specific Protocols
WB protocol for VARS2 antibody 15776-1-APDownload protocol
IHC protocol for VARS2 antibody 15776-1-APDownload protocol
IF protocol for VARS2 antibody 15776-1-APDownload protocol
IP protocol for VARS2 antibody 15776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EMBO Mol Med

The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.

Authors - Elena Perli
View Article
humanIHC

Int J Mol Sci

VARS2 Depletion Leads to Activation of the Integrated Stress Response and Disruptions in Mitochondrial Fatty Acid Oxidation.

Authors - Elham Kayvanpour
  • KD Validated
View Article
humanWB

Front Pediatr

Case Report and Review of the Literature: A New and a Recurrent Variant in the VARS2 Gene Are Associated With Isolated Lethal Hypertrophic Cardiomyopathy, Hyperlactatemia, and Pulmonary Hypertension in Early Infancy.

Authors - Katarína Kušíková
View Article
humanWB

Metab Brain Dis

Neonatal encephalocardiomyopathy caused by mutations in VARS2.

Authors - Fabian Baertling
View Article