CoraLite® Plus 555-conjugated VAPB Polyclonal antibody

• Featured Product
• KD/KO Validated

VAPB Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14477

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

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Validation Data Gallery

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  FC experiment of HepG2 using CL555-14477

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 555-conjugated VAPB Polyclonal antibody (CL555-14477)(red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Jurkat using CL555-14477

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human VAPB (CL555-14477) (red), or 0.2 ug Rabbit IgG CL555-30000(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: Jurkat cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14477 targets VAPB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAPB fusion protein Ag5857
• Full Name: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001712
• Gene Symbol: VAPB
• Gene ID (NCBI): 9217
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.