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  • KD/KO Validated

VAPB Polyclonal antibody

VAPB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 14477-1-AP

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB



Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, HepG2 cells, rat brain tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, mouse liver tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14477-1-AP targets VAPB in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAPB fusion protein Ag5857
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001712
Gene Symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_2288297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Protocols

Product Specific Protocols
WB protocol for VAPB antibody 14477-1-APDownload protocol
IHC protocol for VAPB antibody 14477-1-APDownload protocol
IF protocol for VAPB antibody 14477-1-APDownload protocol
IP protocol for VAPB antibody 14477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
monkeyWB

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
monkeyWB

Mol Cell

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Authors - Christophe A C Freyre
humanWB

J Clin Invest

SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.

Authors - Museer A Lone
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (09-22-2020)

The antibody have predicted band on western blot.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse heart