VAPB Polyclonal antibody

• Featured Product
• KD/KO Validated

VAPB Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

46

Reviews (1)

Cat no : 14477-1-AP

Synonyms

VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983

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Validation Data Gallery

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  WB analysis of HepG2 using 14477-1-AP

  WB result of VAPB antibody (14477-1-AP, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

• 

  WB analysis using 14477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14477-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14477-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14477-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14477-1-AP

  IP result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 14477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAPB antibody (14477-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 14477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VAPB antibody (14477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 14477-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VAPB Polyclonal antibody (14477-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, HepG2 cells, rat brain tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, mouse liver tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 36 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

14477-1-AP targets VAPB in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAPB fusion protein Ag5857
• Full Name: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001712
• Gene Symbol: VAPB
• Gene ID (NCBI): 9217
• RRID: AB_2288297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late-onset spinal muscular atrophy.

Publications

Species	Application	Title
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
monkey	WB	Mol Cell; MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.; Authors - Christophe A C Freyre
human	WB	J Clin Invest; SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.; Authors - Museer A Lone
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (09-22-2020)

The antibody have predicted band on western blot.

• Applications: Western Blot
• Cell Tissue Type: Mouse heart

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