VAMP3/Cellubrevin Monoclonal antibody, PBS Only

VAMP3/Cellubrevin Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D10D10

Cat no : 66488-1-PBS

Synonyms

CEB, Cellubrevin, SYB3, Synaptobrevin 3, VAMP 3, VAMP3, VAMP3/Cellubrevin

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Validation Data Gallery

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  WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

  Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66488-1-Ig (same clone as 66488-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66488-1-PBS targets VAMP3/Cellubrevin in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: VAMP3/Cellubrevin fusion protein Ag1156
• Full Name: vesicle-associated membrane protein 3 (cellubrevin)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC005941
• Gene Symbol: VAMP3
• Gene ID (NCBI): 9341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.