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VAMP3/Cellubrevin Monoclonal antibody

VAMP3/Cellubrevin Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D10D10

Cat no : 66488-1-Ig

Synonyms

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of pig brain tissue using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    WB analysis of pig brain using 66488-1-Ig

    Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    WB analysis of HEK-293 using 66488-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using VAMP3/Cellubrevin Monoclonal antibody (66488-1-Ig)

    IF Staining of Neuro-2a using 66488-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, HEK-293 cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:900-1:3600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66488-1-Ig targets VAMP3/Cellubrevin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen VAMP3/Cellubrevin fusion protein Ag1156
Full Name vesicle-associated membrane protein 3 (cellubrevin)
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC005941
Gene Symbol VAMP3
Gene ID (NCBI) 9341
RRIDAB_2881853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Protocols

Product Specific Protocols
WB protocol for VAMP3/Cellubrevin antibody 66488-1-IgDownload protocol
IF protocol for VAMP3/Cellubrevin antibody 66488-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Long non-coding RNA NORAD promotes the prostate cancer cell extracellular vesicle release via microRNA-541-3p-regulated PKM2 to induce bone metastasis of prostate cancer.

Authors - Chuan-Yi Hu
View Article