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ChromoTek V5-Trap® Magnetic Particles M-270

The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

V5-Tag

Applications

IP, CoIP

Conjugate

Magnetic Particles M-270

Cat no : v5td

Synonyms

Magnetic Particles M-270, V5-Trap Magnetic Particles M-270, V5 tag, V5-tag, V5-Trap®, V5-Trap® Magnetic Particles M-270, v5td, v5td-10, v5td-100, v5td-20, v5td-200, v5td-400

Validation Data Gallery

  • V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. I: Input, FT: Flow-Through, B: Bound.

    V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. I: Input, FT: Flow-Through, B: Bound.

  • V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

    V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

Product Information

The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 40 nM
Specificity/TargetV5-Tag
Coupled VHHRecombinant, monoclonal anti-V5-tag single domain antibody fragment
ConjugateMagnetic Particles M-270
Beads2.8 µm
Binding capacity0.62-1.25 µg of recombinant V5-tagged protein (~30 kDa) per 25 µL bead slurry
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!