Validation Data Gallery
![V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound](https://www.ptglab.com/products/pictures/Result-v5td_20210903110512372.png "V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound")
Product Information
The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
| Description | Immunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to magnetic particles. V5-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.
• No heavy & light antibody chains in downstream applications • Very low background • Effective elution with V5-peptide • Stable under harsh washing conditions • Efficient immunoprecipitation of V5-tag fusion proteins |
| Applications | IP, CoIP, ChIP, RIP, Protein purification |
| Specificity/Target | V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein. |
| Binding capacity | 0.62-1.25 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic Particles M-270, size: 2.8 µm high throughput-compatible |
| Elution buffer | V5-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 % Nonidet P40 Substitute, 2 % Triton X-100, 150 mM NaCl, 10 mM TCEP, 10 mM DTT, 50 mM Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 40 nM |
| Compatibility with mass spectrometry | The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2892994 |
| Storage Buffer | PBS with 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
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| v5td_SDS_V5-Trap® Magnetic Particles M-270 (EN) |
| Datasheet |
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| Protocol V5-Trap Magnetic Particles M-270 |
Publications
| Application | Title |
|---|---|
mBio On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa | |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Magdalena (Verified Customer) (02-12-2025) | The pulldown of V5-tagged protein worked very well with this product. The yield was a lot better than with the magnetic agarose. The only issue was when eluting with LSB (as stated in the protocol), a precipitate or broken resin formed, which was not magnetic anymore and had to be spun down before gel loading.
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FH Karel (Verified Customer) (11-20-2024) | The purity was excellent as we used the sample of masss pecrometry analysis.
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FH Karel (Verified Customer) (03-14-2024) | reproducible purification without background. The purified proteins we want to use for mass spec analysis
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FH Tsimafei (Verified Customer) (12-04-2022) | IP was performed using cells expressing V5-tagged mCherry. 10µl of beads were used for 0.5mg of total protein. Excellent performance!
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