ChromoTek V5-Trap® Magnetic Particles M-270
The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Cat no : v5td
Synonyms
Validation Data Gallery
Product Information
The ChromoTek V5-Trap® Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Applications | IP, CoIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 40 nM |
Specificity/Target | V5-Tag |
Coupled VHH | Recombinant, monoclonal anti-V5-tag single domain antibody fragment |
Conjugate | Magnetic Particles M-270 |
Beads | 2.8 µm |
Binding capacity | 0.62-1.25 µg of recombinant V5-tagged protein (~30 kDa) per 25 µL bead slurry |
Elution buffer | V5-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS V5-Trap Magnetic Particles M-270 |
Protocol |
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Protocol V5-Trap Magnetic Particles M-270 |