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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated USP9X Polyclonal antibody

USP9X Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-55054

Synonyms

Fat facets protein-related, X-linked, FAM, FAF, EC:3.4.19.12, DFFRX

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated USP9X Polyclonal antibody (CL594-55054)

    IF Staining of HeLa using CL594-55054

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 USP9X antibody (CL594-55054) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated USP9X Polyclonal antibody (CL594-55054)

    FC experiment of HeLa using CL594-55054

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human USP9X (CL594-55054) (red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-55054 targets USP9X in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ubiquitin specific peptidase 9, X-linked
Calculated Molecular Weight 292 kDa
Observed Molecular Weight 260-290 kDa
GenBank Accession NumberNM_001039590
Gene Symbol USP9X
Gene ID (NCBI) 8239
RRIDAB_2919884
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

Protocols

Product Specific Protocols
IF protocol for CL594 USP9X antibody CL594-55054Download protocol
Standard Protocols
Click here to view our Standard Protocols