Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 USP7 antibody (CL488-66514, Clone: 3D7D11 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 USP7 antibody (CL488-66514, Clone: 3D7D11 ) at dilution of 1:200.
FC experiment of HeLa using CL488-66514
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USP7 (CL488-66514, Clone:3D7D11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USP7 (CL488-66514, Clone:3D7D11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 USP7 antibody (CL488-66514, Clone: 3D7D11 ) at dilution of 1:200.
FC (INTRA) Figures
FC experiment of HeLa using CL488-66514
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USP7 (CL488-66514, Clone:3D7D11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
USP7 Monoclonal antibody
Catalog Number
CL488-66514
Citations
-
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
