USP5 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

USP5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15158-1-PBS

Synonyms

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Validation Data Gallery

• 

  WB analysis of mouse lung using 15158-1-AP (same clone as 15158-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 15158-1-AP (same clone as 15158-1-PBS)

  WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 15158-1-AP (same clone as 15158-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 15158-1-AP (same clone as 15158-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15158-1-AP (same clone as 15158-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15158-1-AP (same clone as 15158-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-453s using 15158-1-AP (same clone as 15158-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15158-1-AP (same clone as 15158-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 15158-1-AP (same clone as 15158-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat lung using 15158-1-AP (same clone as 15158-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 15158-1-AP (same clone as 15158-1-PBS)

  IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15158-1-PBS in a different storage buffer formulation.

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Product Information

15158-1-PBS targets USP5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP5 fusion protein Ag7252
• Full Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC005139
• Gene Symbol: USP5
• Gene ID (NCBI): 8078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.