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USP5 Polyclonal antibody

USP5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15158-1-AP

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of A549 using 15158-1-AP

    WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.

  • Western Blot (WB) analysis of mouse lung tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of mouse lung using 15158-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of rat brain using 15158-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of rat lung using 15158-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of HeLa using 15158-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of human brain using 15158-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of human brain using 15158-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of HeLa using 15158-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of HeLa using 15158-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using USP5 Polyclonal antibody (15158-1-AP)

    WB analysis of MDA-MB-453s using 15158-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using USP5 Polyclonal antibody (15158-1-AP)

    IP experiment of mouse brain using 15158-1-AP

    IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, HeLa cells, human brain tissue, rat lung tissue, rat brain tissue, MDA-MB-453s cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15158-1-AP targets USP5 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP5 fusion protein Ag7252
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC005139
Gene Symbol USP5
Gene ID (NCBI) 8078
RRIDAB_2213856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Protocols

Product Specific Protocols
WB protocol for USP5 antibody 15158-1-APDownload protocol
IP protocol for USP5 antibody 15158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IP, IF

Neuron

The Deubiquitinating Enzyme USP5 Modulates Neuropathic and Inflammatory Pain by Enhancing Cav3.2 Channel Activity.

Authors - Agustin García-Caballero
View Article
WB

FASEB J

PPP2R1B is modulated by ubiquitination and is essential for spermatogenesis.

Authors - Mufeng Du
View Article
mouseWB

Toxicology

Critical role of Cav3.2 T-type calcium channels in the peripheral neuropathy induced by bortezomib, a proteasome-inhibiting chemotherapeutic agent, in mice.

Authors - Shiori Tomita
View Article