CoraLite® Plus 555-conjugated USP14 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP14 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14517

Synonyms

USP14, Deubiquitinating enzyme 14, EC:3.4.19.12, TGT, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin thioesterase 14, Ubiquitin thiolesterase 14, Ubiquitin-specific-processing protease 14, USP 14

---

Validation Data Gallery

• 

  FC experiment of HepG2 using CL555-14517

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human USP14 (CL555-14517) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive FC (Intra) detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14517 targets USP14 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP14 fusion protein Ag5979
• Full Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: BC003556
• Gene Symbol: USP14
• Gene ID (NCBI): 9097
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).