CoraLite®594-conjugated USP11 Monoclonal antibody

USP11 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G6E7

Cat no : CL594-67805

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67805

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 USP11 antibody (CL594-67805, Clone: 2G6E7 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67805 targets USP11 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP11 fusion protein Ag19058
• Full Name: ubiquitin specific peptidase 11
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC000350
• Gene Symbol: USP11
• Gene ID (NCBI): 8237
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.