USP1 Monoclonal antibody

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  WB analysis of HepG2 using 66069-1-Ig

  WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells.

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  WB analysis of HepG2 using 66069-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66069-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66069-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66069-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66069-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 66069-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

USP1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66069-1-Ig

Clone No.1C10H6

Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66069-1-Ig targets USP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, monkey
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP1 fusion protein Ag5904
• Full Name: ubiquitin specific peptidase 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC050525
• Gene Symbol: USP1
• Gene ID (NCBI): 7398
• RRID: AB_11043340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94782
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).

Publications

Species	Application	Title
human,monkey	WB	J Virol; Ubiquitin-specific proteinase 1 stabilizes PRRSV nonstructural protein Nsp1β to promote viral replication by regulating K48 ubiquitination; Authors - Yunyun Zhai ; KD Validated
human	IF	Cell Death Differ; Ubiquitin-specific protease 1 facilitates hepatocellular carcinoma progression by modulating mitochondrial fission and metabolic reprogramming via cyclin-dependent kinase 5 stabilization; Authors - Saiyan Bian