UQCRFS1 Monoclonal antibody

UQCRFS1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68529-1-Ig
Clone No.1F11C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Cytochrome b-c1 complex subunit Rieske, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 5, Complex III subunit IX, Complex III subunit 5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68529-1-Ig targets UQCRFS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UQCRFS1 fusion protein Ag13307 Predict reactive species
Full Name ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC010035
Gene Symbol UQCRFS1
Gene ID (NCBI) 7386
RRIDAB_3085237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP47985
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.

Protocols

Product Specific Protocols
WB protocol for UQCRFS1 antibody 68529-1-IgDownload protocol
IF protocol for UQCRFS1 antibody 68529-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Cancerous Conditions Accelerate the Aging of Skeletal Muscle via Mitochondrial DNA Damage

Authors - Yi Luo
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