UQCRFS1 Monoclonal antibody, PBS Only

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  WB analysis using 68529-1-Ig (same clone as 68529-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68529-1-Ig (UQCRFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68529-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68529-1-Ig (same clone as 68529-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UQCRFS1 antibody (68529-1-Ig, Clone: 1F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68529-1-PBS in a different storage buffer formulation.

UQCRFS1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68529-1-PBS

Clone No.1F11C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Cytochrome b-c1 complex subunit Rieske, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 5, Complex III subunit IX, Complex III subunit 5

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Product Information

68529-1-PBS targets UQCRFS1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UQCRFS1 fusion protein Ag13307
• Full Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010035
• Gene Symbol: UQCRFS1
• Gene ID (NCBI): 7386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P47985
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.