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UQCRC2 Recombinant antibody, PBS Only

UQCRC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240454D9

Cat no : 83667-2-PBS

Synonyms

Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Recombinant antibody (83667-2-RR)

    WB analysis using 83667-2-RR (same clone as 83667-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83667-2-RR (UQCRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using UQCRC2 Recombinant antibody (83667-2-RR)

    FC experiment of HepG2 using 83667-2-RR (same clone as 83667-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug UQCRC2 Recombinant antibody (83667-2-RR, Clone:240454D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83667-2-RR

    Affinity and Kinetic Characterization of 83667-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83667-2-RR against Human UQCRC2 were performed. The affinity constant is 21.8 nM.

Product Information

The immunogen of 83667-2-PBS is UQCRC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.