UQCRC1 Recombinant antibody

UQCRC1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240507A6

Cat no : 83671-2-RR

Synonyms

QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1

---

Validation Data Gallery

• 

  WB analysis using 83671-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 83671-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83671-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

---

Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83671-2-RR is UQCRC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UQCRC1 fusion protein Ag16322
• Full Name: ubiquinol-cytochrome c reductase core protein I
• Calculated Molecular Weight: 480 aa, 53 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009586
• Gene Symbol: UQCRC1
• Gene ID (NCBI): 7384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.