UQCRC1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83671-2-RR (same clone as 83671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83671-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83671-2-RR (same clone as 83671-2-PBS)

  WB result of UQCRC1 antibody (83671-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UQCRC1 transfected HeLa cells. This data was developed using the same antibody clone with 83671-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83671-2-RR (same clone as 83671-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83671-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83671-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

• Featured Product
• KD/KO Validated

UQCRC1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83671-2-PBS

Clone No.240507A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

240507A6, Complex III subunit 1, Core protein I, Cytochrome b-c1 complex subunit 1, mitochondrial, D3S3191

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Product Information

83671-2-PBS targets UQCRC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16322

  Product name: Recombinant human UQCRC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 159-480 aa of BC009586

  Sequence: SQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF

• Full Name: ubiquinol-cytochrome c reductase core protein I
• Calculated Molecular Weight: 480 aa, 53 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009586
• Gene Symbol: UQCRC1
• Gene ID (NCBI): 7384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31930
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.