UFD1L Recombinant antibody

UFD1L Uni-rAb^TM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230411A2

Cat no : 83669-1-RR

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2

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Validation Data Gallery

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  FC experiment of HEK-293 using 83669-1-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 83669-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83669-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83669-1-RR against Human UFD1L were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC (Intra) detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83669-1-RR is UFD1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UFD1L fusion protein Ag0954
• Full Name: ubiquitin fusion degradation 1 like (yeast)
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: BC005087
• Gene Symbol: UFD1L
• Gene ID (NCBI): 7353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.