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UCP2 Monoclonal antibody, PBS Only

UCP2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B4B1

Cat no : 66700-1-PBS

Synonyms

BMIQ4, SLC25A8, UCP 2, UCP2, UCPH

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using UCP2 Monoclonal antibody (66700-1-Ig)

    WB analysis of Jurkat using 66700-1-Ig (same clone as 66700-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adipose tissue using UCP2 Monoclonal antibody (66700-1-Ig)

    WB analysis of human adipose using 66700-1-Ig (same clone as 66700-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using UCP2 Monoclonal antibody (66700-1-Ig)

    WB analysis of human skeletal muscle using 66700-1-Ig (same clone as 66700-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66700-1-PBS targets UCP2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen UCP2 fusion protein Ag19361
Full Name uncoupling protein 2 (mitochondrial, proton carrier)
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC011737
Gene Symbol UCP2
Gene ID (NCBI) 7351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.