UCP2 Monoclonal antibody

UCP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B4B1

Cat no : 66700-1-Ig

Synonyms

BMIQ4, SLC25A8, UCP 2, UCP2, UCPH

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Validation Data Gallery

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  WB analysis of Jurkat using 66700-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adipose using 66700-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66700-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, human adipose tissue, human skeletal muscle tissue

It is strongly recommended that fresh-made lysate be used for western blot assays.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66700-1-Ig targets UCP2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: UCP2 fusion protein Ag19361
• Full Name: uncoupling protein 2 (mitochondrial, proton carrier)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC011737
• Gene Symbol: UCP2
• Gene ID (NCBI): 7351
• RRID: AB_2882053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.

Publications

Species	Application	Title
human	WB	Phytomedicine; Genipin ameliorates diabetic retinopathy via the HIF-1α and AGEs-RAGE pathways; Authors - Kexin Sun