UCK1 Polyclonal antibody, PBS Only

UCK1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12271-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12271-1-AP (same clone as 12271-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

  IP result of anti-UCK1 (IP:12271-1-AP, 4ug; Detection:12271-1-AP 1:1000) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 12271-1-AP (UCK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

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Product Information

12271-1-PBS targets UCK1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCK1 fusion protein Ag2912
• Full Name: uridine-cytidine kinase 1
• Calculated Molecular Weight: 277 aa, 31 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC015547
• Gene Symbol: UCK1
• Gene ID (NCBI): 83549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.