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UCK1 Polyclonal antibody

UCK1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12271-1-AP

Synonyms

Uridine-cytidine kinase 1, Uridine monophosphokinase 1, uridine cytidine kinase 1, UCK 1, EC:2.7.1.48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis using 12271-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis of MCF-7 using 12271-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis of MCF-7 using 12271-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis of MCF-7 using 12271-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    IP experiment of MCF-7 using 12271-1-AP

    IP result of anti-UCK1 (IP:12271-1-AP, 4ug; Detection:12271-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    IF Staining of MCF-7 using 12271-1-AP

    Immunofluorescent analysis of MCF-7 cells using 12271-1-AP (UCK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12271-1-AP targets UCK1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCK1 fusion protein Ag2912
Full Name uridine-cytidine kinase 1
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC015547
Gene Symbol UCK1
Gene ID (NCBI) 83549
RRIDAB_11182173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.

Protocols

Product Specific Protocols
WB protocol for UCK1 antibody 12271-1-APDownload protocol
IF protocol for UCK1 antibody 12271-1-APDownload protocol
IP protocol for UCK1 antibody 12271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Cancer

Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.

Authors - Nao Yoshida-Sakai
View Article
human,mouseWB

Blood Adv

Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells

Authors - Tatsuro Watanabe
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

H (Verified Customer) (07-09-2020)

This antibody is good considering the low expression of UCK1 protein. I could see a thick band that corresponds with UCK1 MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
UCK1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:12271-1-AP)