UCH-L1/PGP9.5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

UCH-L1/PGP9.5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C9E11

Cat no : 66230-1-PBS

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

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Validation Data Gallery

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  WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66230-1-Ig (same clone as 66230-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UCHL1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

  1x10^6 Y79 cells were intracellularly stained with 0.4 ug UCHL1/PGP9.5 Monoclonal antibody (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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Product Information

66230-1-PBS targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6547
• Full Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000332
• Gene Symbol: UCHL1
• Gene ID (NCBI): 7345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)