UBR2 Monoclonal antibody

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  WB analysis using 68565-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68565-1-Ig (UBR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

UBR2 Monoclonal Antibody for WB, ELISA

Cat No. 68565-1-Ig

Clone No.1D5H2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0349, EC:2.3.2.27, E3 ubiquitin-protein ligase UBR2, C6orf133, bA49A4.1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68565-1-Ig targets UBR2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBR2 fusion protein Ag13446
• Full Name: ubiquitin protein ligase E3 component n-recognin 2
• Calculated Molecular Weight: 439aa,50 kDa; 1755aa,201 kDa
• Observed Molecular Weight: 201 kDa
• GenBank Accession Number: BC064512
• Gene Symbol: UBR2
• Gene ID (NCBI): 23304
• RRID: AB_3085267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8IWV8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.