UBE2S Monoclonal antibody, PBS Only

UBE2S Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C9E6

Cat no : 66897-1-PBS

Synonyms

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

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Validation Data Gallery

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  WB analysis of HEK-293 using 66897-1-Ig (same clone as 66897-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66897-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66897-1-Ig (same clone as 66897-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66897-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66897-1-Ig (same clone as 66897-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66897-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66897-1-PBS targets UBE2S in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBE2S fusion protein Ag5378
• Full Name: ubiquitin-conjugating enzyme E2S
• Calculated Molecular Weight: 222 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC066948
• Gene Symbol: UBE2S
• Gene ID (NCBI): 27338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).