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UBE2S Monoclonal antibody, PBS Only

UBE2S Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C9E6

Cat no : 66897-1-PBS

Synonyms

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using UBE2S Monoclonal antibody (66897-1-Ig)

    WB analysis of HEK-293 using 66897-1-Ig (same clone as 66897-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using UBE2S Monoclonal antibody (66897-1-Ig)

    WB analysis of Jurkat using 66897-1-Ig (same clone as 66897-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UBE2S Monoclonal antibody (66897-1-Ig)

    WB analysis of HEK-293 using 66897-1-Ig (same clone as 66897-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66897-1-PBS in a different storage buffer formulation.

Product Information

66897-1-PBS targets UBE2S in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen UBE2S fusion protein Ag5378
Full Name ubiquitin-conjugating enzyme E2S
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).