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UBC9-Specific Monoclonal antibody, PBS Only

UBC9-Specific Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8A2

Cat no : 67660-1-PBS

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, UBE2I-Specific, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBC9-Specific Monoclonal antibody (67660-1-Ig)

    WB analysis using 67660-1-Ig (same clone as 67660-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67660-1-Ig (UBC9-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67660-1-PBS in a different storage buffer formulation.

Product Information

67660-1-PBS targets UBC9-Specific in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberNM_003345
Gene Symbol UBC9
Gene ID (NCBI) 7329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).