UBAC1 Monoclonal antibody, PBS Only

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  WB analysis using 67385-1-Ig (same clone as 67385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67385-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67385-1-Ig (same clone as 67385-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67385-1-PBS in a different storage buffer formulation.

UBAC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67385-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1

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Product Information

67385-1-PBS targets UBAC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBAC1 fusion protein Ag13958
• Full Name: UBA domain containing 1
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC004967
• Gene Symbol: UBAC1
• Gene ID (NCBI): 10422
• RRID: AB_2882630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BSL1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.