Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF Staining of HeLa using 67385-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.
Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF/ICC Figures
IF Staining of HeLa using 67385-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
UBAC1 Monoclonal antibody
Catalog Number
67385-1-Ig
Citations
1
Dilutions
WB : 1:5000-1:50000 IF/ICC : 1:400-1:1600
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
