U2AF35 Monoclonal antibody, PBS Only

U2AF35 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7F11

Cat no : 60289-1-PBS

Synonyms

U2AF1, U2AFBP, RNU2AF1, RN, FP793

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Validation Data Gallery

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  WB analysis of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

  WB result of U2AF35 antibody (60289-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  WB analysis using 60289-1-Ig (same clone as 60289-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60289-1-Ig (same clone as 60289-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

  IP result of anti-U2AF35 (IP:60289-1-Ig, 3ug; Detection:60289-1-Ig 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 60289-1-Ig (same clone as 60289-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human U2AF35 (60289-1-Ig, Clone:2B7F11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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Product Information

60289-1-PBS targets U2AF35 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: U2AF35 fusion protein Ag0399
• Full Name: U2 small nuclear RNA auxiliary factor 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC001923
• Gene Symbol: U2AF1
• Gene ID (NCBI): 7307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.