Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis of 4T1 using 68166-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 68166-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 68166-1-Ig
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 68166-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HEK-293 using 68166-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HEK-293 using 68166-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC experiment of HEK-293 using 68166-1-Ig
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human U2AF2 (68166-1-Ig, Clone:1B7E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human U2AF2 (68166-1-Ig, Clone:1B7E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells
Positive FC (Intra) detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
U2AF2, also named as U2 auxiliary factor 65 kDa subunit, is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis of 4T1 using 68166-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse liver using 68166-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 68166-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 68166-1-Ig
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HEK-293 using 68166-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HEK-293 using 68166-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HEK-293 using 68166-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HEK-293 using 68166-1-Ig
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human U2AF2 (68166-1-Ig, Clone:1B7E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
U2AF2/U2AF65 Monoclonal antibody
Catalog Number
68166-1-Ig
Citations
1
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
